This week we discuss our plan of having three pools sequenced and next steps going forward. Three pools for the current three libraries are as follows:
uninfected from Day 9 (before temperature treatment began)
infected from Day 9 (before temperature treatment began)
“MasterPool” : 10 samples. One sample per each of the following treatments:
uninfected, day 12, cold
infected, day 12, cold
uninfected, day 12, ambient
infected, day 12, ambient
uninfected, day 12, warm
infected, day 12, warm
uninfected, day 26, cold
infected, day 26, cold
uninfected, day 26, ambient
infected, day 26, ambient
This week I speak with Genevieve Johnson, a MS student at University of Alaska, Fairbanks. We talk about her thesis work on population genetics of Alaskan Tanner Crabs, her experience taking crab samples on a vessel, and her thoughts on her future.
This week is Part 2 of FAQ with Pam. We talk about:
Mass die-off in warm treatment crabs
Signs vs symptoms of BCS (Bitter Crab Syndrome/Bitter Crab Disease)
This week we discuss R scripts for combining files, qPCR results for infection status, and next steps.
This week we talk about the problems Tanner crabs face (Bitter Crab Disease) as well as what our project is all about.